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plasmid pet-22b  (Millipore)


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    Structured Review

    Millipore plasmid pet-22b
    Plasmid Pet 22b, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+pet-22b/pet22b/pmc12131743-163-0-5
    Average 90 stars, based on 1 article reviews
    plasmid pet-22b - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Plasmid Preparation:


    Article Title: Immobilization of a Novel ESTBAS Esterase from Bacillus altitudinis onto an Epoxy Resin: Characterization and Regioselective Synthesis of Chloramphenicol Palmitate
    Article Snippet: Plasmid pET-22b (Novagen, Madison, WI, USA) served as a vector to construct the protein expression plasmid for E. coli BL21 (DE3).

    Article Title: Influence of production process design on inclusion bodies protein: the case of an Antarctic flavohemoglobin
    Article Snippet: Plasmid pET-22b (Novagen) was utilized for cloning and expression.

    Article Title: Comparison of Enzyme Secretion and Ferulic Acid Production by Escherichia coli Expressing Different Lactobacillus Feruloyl Esterases
    Article Snippet: Plasmid pET-22b (Novagen, Madison, United States) was used for the ligation of feruloyl esterase genes.

    Article Title: Growth advantage of Escherichia coli O104:H4 strains on 5-N-acetyl-9-O-acetyl neuraminic acid as a carbon source is dependent on heterogeneous phage-Borne nanS-p esterases.
    Article Snippet: Enterohemorrhagic E. coli (EHEC) are serious bacterial pathogens which are able to cause a hemorrhagic colitis or the life-threatening hemolytic-uremic syndrome (HUS) in humans.. EHEC strains can carry different numbers of phage-borne nanS-p alleles that are responsible for acetic acid release from mucin from bovine submaxillary gland and 5-N-acetyl-9-O-acetyl neuraminic acid (Neu5,9Ac2), a carbohydrate present in mucin.. Thus, Neu5,9Ac2 can be transformed to 5-N-acetyl neuraminic acid, an energy source used by E. coli strains.

    Article Title: Detection of human annexin A1 as the novel N-terminal tag for separation and purification handle
    Article Snippet: E. coli strains DH5α and BL21 (DE3), and the plasmid pET-22b and pET-28b are products of Novagen (USA).

    Article Title: Living materials fabricated via gradient mineralization of light-inducible biofilms.
    Article Snippet: 1Materials and Physical Biology Division, School of Physical Science and Technology, ShanghaiTech University, Shanghai, China.. 2Collaborative Innovation Center of Advanced Microstructures, National Laboratory of Solid State Microstructure, Department of Physics, Nanjing University, Nanjing, China.. 3Center for Materials Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.

    Article Title: Contrast agent for photoacoustic imaging
    Article Snippet: A plasmid pET-22b (+) (Novagen) in which the foregoing gene fragment was inserted downstream of the T7 promoter was introduced into Escherichia coli BL21 (DE3) to give a strain for expression.

    Clone Assay:


    Article Title: Immobilization of a Novel ESTBAS Esterase from Bacillus altitudinis onto an Epoxy Resin: Characterization and Regioselective Synthesis of Chloramphenicol Palmitate
    Article Snippet: Plasmid pET-22b (Novagen, Madison, WI, USA) served as a vector to construct the protein expression plasmid for E. coli BL21 (DE3).

    Article Title: Influence of production process design on inclusion bodies protein: the case of an Antarctic flavohemoglobin
    Article Snippet: Plasmid pET-22b (Novagen) was utilized for cloning and expression.

    Article Title: Comparison of Enzyme Secretion and Ferulic Acid Production by Escherichia coli Expressing Different Lactobacillus Feruloyl Esterases
    Article Snippet: Plasmid pET-22b (Novagen, Madison, United States) was used for the ligation of feruloyl esterase genes.

    Article Title: Growth advantage of Escherichia coli O104:H4 strains on 5-N-acetyl-9-O-acetyl neuraminic acid as a carbon source is dependent on heterogeneous phage-Borne nanS-p esterases.
    Article Snippet: Enterohemorrhagic E. coli (EHEC) are serious bacterial pathogens which are able to cause a hemorrhagic colitis or the life-threatening hemolytic-uremic syndrome (HUS) in humans.. EHEC strains can carry different numbers of phage-borne nanS-p alleles that are responsible for acetic acid release from mucin from bovine submaxillary gland and 5-N-acetyl-9-O-acetyl neuraminic acid (Neu5,9Ac2), a carbohydrate present in mucin.. Thus, Neu5,9Ac2 can be transformed to 5-N-acetyl neuraminic acid, an energy source used by E. coli strains.

    Article Title: Detection of human annexin A1 as the novel N-terminal tag for separation and purification handle
    Article Snippet: E. coli strains DH5α and BL21 (DE3), and the plasmid pET-22b and pET-28b are products of Novagen (USA).

    Article Title: Living materials fabricated via gradient mineralization of light-inducible biofilms.
    Article Snippet: 1Materials and Physical Biology Division, School of Physical Science and Technology, ShanghaiTech University, Shanghai, China.. 2Collaborative Innovation Center of Advanced Microstructures, National Laboratory of Solid State Microstructure, Department of Physics, Nanjing University, Nanjing, China.. 3Center for Materials Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.

    Article Title: Contrast agent for photoacoustic imaging
    Article Snippet: A plasmid pET-22b (+) (Novagen) in which the foregoing gene fragment was inserted downstream of the T7 promoter was introduced into Escherichia coli BL21 (DE3) to give a strain for expression.

    Expressing:


    Article Title: Immobilization of a Novel ESTBAS Esterase from Bacillus altitudinis onto an Epoxy Resin: Characterization and Regioselective Synthesis of Chloramphenicol Palmitate
    Article Snippet: Plasmid pET-22b (Novagen, Madison, WI, USA) served as a vector to construct the protein expression plasmid for E. coli BL21 (DE3).

    Article Title: Influence of production process design on inclusion bodies protein: the case of an Antarctic flavohemoglobin
    Article Snippet: Plasmid pET-22b (Novagen) was utilized for cloning and expression.

    Article Title: Comparison of Enzyme Secretion and Ferulic Acid Production by Escherichia coli Expressing Different Lactobacillus Feruloyl Esterases
    Article Snippet: Plasmid pET-22b (Novagen, Madison, United States) was used for the ligation of feruloyl esterase genes.

    Article Title: Growth advantage of Escherichia coli O104:H4 strains on 5-N-acetyl-9-O-acetyl neuraminic acid as a carbon source is dependent on heterogeneous phage-Borne nanS-p esterases.
    Article Snippet: Enterohemorrhagic E. coli (EHEC) are serious bacterial pathogens which are able to cause a hemorrhagic colitis or the life-threatening hemolytic-uremic syndrome (HUS) in humans.. EHEC strains can carry different numbers of phage-borne nanS-p alleles that are responsible for acetic acid release from mucin from bovine submaxillary gland and 5-N-acetyl-9-O-acetyl neuraminic acid (Neu5,9Ac2), a carbohydrate present in mucin.. Thus, Neu5,9Ac2 can be transformed to 5-N-acetyl neuraminic acid, an energy source used by E. coli strains.

    Article Title: Detection of human annexin A1 as the novel N-terminal tag for separation and purification handle
    Article Snippet: E. coli strains DH5α and BL21 (DE3), and the plasmid pET-22b and pET-28b are products of Novagen (USA).

    Article Title: Living materials fabricated via gradient mineralization of light-inducible biofilms.
    Article Snippet: 1Materials and Physical Biology Division, School of Physical Science and Technology, ShanghaiTech University, Shanghai, China.. 2Collaborative Innovation Center of Advanced Microstructures, National Laboratory of Solid State Microstructure, Department of Physics, Nanjing University, Nanjing, China.. 3Center for Materials Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.

    Article Title: Contrast agent for photoacoustic imaging
    Article Snippet: A plasmid pET-22b (+) (Novagen) in which the foregoing gene fragment was inserted downstream of the T7 promoter was introduced into Escherichia coli BL21 (DE3) to give a strain for expression.

    Ligation:


    Article Title: Immobilization of a Novel ESTBAS Esterase from Bacillus altitudinis onto an Epoxy Resin: Characterization and Regioselective Synthesis of Chloramphenicol Palmitate
    Article Snippet: Plasmid pET-22b (Novagen, Madison, WI, USA) served as a vector to construct the protein expression plasmid for E. coli BL21 (DE3).

    Article Title: Influence of production process design on inclusion bodies protein: the case of an Antarctic flavohemoglobin
    Article Snippet: Plasmid pET-22b (Novagen) was utilized for cloning and expression.

    Article Title: Comparison of Enzyme Secretion and Ferulic Acid Production by Escherichia coli Expressing Different Lactobacillus Feruloyl Esterases
    Article Snippet: Plasmid pET-22b (Novagen, Madison, United States) was used for the ligation of feruloyl esterase genes.

    Article Title: Growth advantage of Escherichia coli O104:H4 strains on 5-N-acetyl-9-O-acetyl neuraminic acid as a carbon source is dependent on heterogeneous phage-Borne nanS-p esterases.
    Article Snippet: Enterohemorrhagic E. coli (EHEC) are serious bacterial pathogens which are able to cause a hemorrhagic colitis or the life-threatening hemolytic-uremic syndrome (HUS) in humans.. EHEC strains can carry different numbers of phage-borne nanS-p alleles that are responsible for acetic acid release from mucin from bovine submaxillary gland and 5-N-acetyl-9-O-acetyl neuraminic acid (Neu5,9Ac2), a carbohydrate present in mucin.. Thus, Neu5,9Ac2 can be transformed to 5-N-acetyl neuraminic acid, an energy source used by E. coli strains.

    Article Title: Detection of human annexin A1 as the novel N-terminal tag for separation and purification handle
    Article Snippet: E. coli strains DH5α and BL21 (DE3), and the plasmid pET-22b and pET-28b are products of Novagen (USA).

    Article Title: Living materials fabricated via gradient mineralization of light-inducible biofilms.
    Article Snippet: 1Materials and Physical Biology Division, School of Physical Science and Technology, ShanghaiTech University, Shanghai, China.. 2Collaborative Innovation Center of Advanced Microstructures, National Laboratory of Solid State Microstructure, Department of Physics, Nanjing University, Nanjing, China.. 3Center for Materials Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.

    Article Title: Contrast agent for photoacoustic imaging
    Article Snippet: A plasmid pET-22b (+) (Novagen) in which the foregoing gene fragment was inserted downstream of the T7 promoter was introduced into Escherichia coli BL21 (DE3) to give a strain for expression.

    Derivative Assay:


    Article Title: Immobilization of a Novel ESTBAS Esterase from Bacillus altitudinis onto an Epoxy Resin: Characterization and Regioselective Synthesis of Chloramphenicol Palmitate
    Article Snippet: Plasmid pET-22b (Novagen, Madison, WI, USA) served as a vector to construct the protein expression plasmid for E. coli BL21 (DE3).

    Article Title: Influence of production process design on inclusion bodies protein: the case of an Antarctic flavohemoglobin
    Article Snippet: Plasmid pET-22b (Novagen) was utilized for cloning and expression.

    Article Title: Comparison of Enzyme Secretion and Ferulic Acid Production by Escherichia coli Expressing Different Lactobacillus Feruloyl Esterases
    Article Snippet: Plasmid pET-22b (Novagen, Madison, United States) was used for the ligation of feruloyl esterase genes.

    Article Title: Growth advantage of Escherichia coli O104:H4 strains on 5-N-acetyl-9-O-acetyl neuraminic acid as a carbon source is dependent on heterogeneous phage-Borne nanS-p esterases.
    Article Snippet: Enterohemorrhagic E. coli (EHEC) are serious bacterial pathogens which are able to cause a hemorrhagic colitis or the life-threatening hemolytic-uremic syndrome (HUS) in humans.. EHEC strains can carry different numbers of phage-borne nanS-p alleles that are responsible for acetic acid release from mucin from bovine submaxillary gland and 5-N-acetyl-9-O-acetyl neuraminic acid (Neu5,9Ac2), a carbohydrate present in mucin.. Thus, Neu5,9Ac2 can be transformed to 5-N-acetyl neuraminic acid, an energy source used by E. coli strains.

    Article Title: Detection of human annexin A1 as the novel N-terminal tag for separation and purification handle
    Article Snippet: E. coli strains DH5α and BL21 (DE3), and the plasmid pET-22b and pET-28b are products of Novagen (USA).

    Article Title: Living materials fabricated via gradient mineralization of light-inducible biofilms.
    Article Snippet: 1Materials and Physical Biology Division, School of Physical Science and Technology, ShanghaiTech University, Shanghai, China.. 2Collaborative Innovation Center of Advanced Microstructures, National Laboratory of Solid State Microstructure, Department of Physics, Nanjing University, Nanjing, China.. 3Center for Materials Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.

    Article Title: Contrast agent for photoacoustic imaging
    Article Snippet: A plasmid pET-22b (+) (Novagen) in which the foregoing gene fragment was inserted downstream of the T7 promoter was introduced into Escherichia coli BL21 (DE3) to give a strain for expression.

    Construct:


    Article Title: Immobilization of a Novel ESTBAS Esterase from Bacillus altitudinis onto an Epoxy Resin: Characterization and Regioselective Synthesis of Chloramphenicol Palmitate
    Article Snippet: Plasmid pET-22b (Novagen, Madison, WI, USA) served as a vector to construct the protein expression plasmid for E. coli BL21 (DE3).

    Article Title: Influence of production process design on inclusion bodies protein: the case of an Antarctic flavohemoglobin
    Article Snippet: Plasmid pET-22b (Novagen) was utilized for cloning and expression.

    Article Title: Comparison of Enzyme Secretion and Ferulic Acid Production by Escherichia coli Expressing Different Lactobacillus Feruloyl Esterases
    Article Snippet: Plasmid pET-22b (Novagen, Madison, United States) was used for the ligation of feruloyl esterase genes.

    Article Title: Growth advantage of Escherichia coli O104:H4 strains on 5-N-acetyl-9-O-acetyl neuraminic acid as a carbon source is dependent on heterogeneous phage-Borne nanS-p esterases.
    Article Snippet: Enterohemorrhagic E. coli (EHEC) are serious bacterial pathogens which are able to cause a hemorrhagic colitis or the life-threatening hemolytic-uremic syndrome (HUS) in humans.. EHEC strains can carry different numbers of phage-borne nanS-p alleles that are responsible for acetic acid release from mucin from bovine submaxillary gland and 5-N-acetyl-9-O-acetyl neuraminic acid (Neu5,9Ac2), a carbohydrate present in mucin.. Thus, Neu5,9Ac2 can be transformed to 5-N-acetyl neuraminic acid, an energy source used by E. coli strains.

    Article Title: Detection of human annexin A1 as the novel N-terminal tag for separation and purification handle
    Article Snippet: E. coli strains DH5α and BL21 (DE3), and the plasmid pET-22b and pET-28b are products of Novagen (USA).

    Article Title: Living materials fabricated via gradient mineralization of light-inducible biofilms.
    Article Snippet: 1Materials and Physical Biology Division, School of Physical Science and Technology, ShanghaiTech University, Shanghai, China.. 2Collaborative Innovation Center of Advanced Microstructures, National Laboratory of Solid State Microstructure, Department of Physics, Nanjing University, Nanjing, China.. 3Center for Materials Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.

    Article Title: Contrast agent for photoacoustic imaging
    Article Snippet: A plasmid pET-22b (+) (Novagen) in which the foregoing gene fragment was inserted downstream of the T7 promoter was introduced into Escherichia coli BL21 (DE3) to give a strain for expression.



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